Schematic representation illustrating changes in microtubule and actin networks resulting from genetic and pharmacological perturbations. Top: Wild-type cells with normal microtubule (light orange) and actin (blue) networks. Bottom left: Knocking out profilin 1 or exposure to latrunculin A (LatA) leads to increase in both the number, acetylation (dark orange), and stabilization of microtubules, as well as a reduction in F-actin. Bottom right: Treatment with the myosin inhibitor blebbistatin leads to more microtubules and increased microtubule acetylation without affecting the total amount of filamentous actin. MT: microtubule.