Irf8+32^−/− mice specifically lack cDC1s during Cryptosporidium infection. (A) WT or Irf8+32^−/− mice were left uninfected or infected with 5 × 10⁴ maCp oocysts. At 3 dpi, mice were sacrificed and percentage of cDC1s and cDC2s were quantified to confirm that cDC1s remained deficient in Irf8+32^−/− mice. Gating: Singlets, Live⁺, CD3e⁻, NK1.1⁻, EpCAM⁻, B220⁻, CD19⁻, CD90.2⁻, CD64⁻, MHCII-hi, CD11c⁺, CD26⁺, Ly6C⁻, XCR1⁺ (cDC1s), or SIRPα⁺ (cDC2s). Shown is one independent experiment representative of two independent experiments, n = 3 mice/group. Bar graphs are plotted as mean with SEM. Statistical significance was determined by two-way ANOVA and multiple comparisons. ***P ≤ 0.001, ****P ≤ 0.0001.