Time to biorientation after STLC washout varies widely between cells. (A) Raw time of chromosome alignment following STLC washout experiments for short and long chromosomes where colors correspond to chromosomes coming from the same cell (n = 9 cells; mean ± SD). (B) Raw time of chromosome alignment in two independent cells from A (example #1 black, and example #2 gray; left) and a zoomed-in plot for ex. #2 (right) demonstrating that after drug washout, cells took variable amounts of time to achieve chromosome alignment initially (mean ± SD). (C) Time of first detectable oscillating chromosome following STLC washout and last to begin oscillating prior to anaphase within the same cell, showing that while there is variability in the duration of the error correction process (∼15-min spread the time between the first and last chromosome alignment), most variability arises in the time between drug washout and the time of first chromosome alignment (∼30-min spread). This variable time before beginning correction of attachment errors following STLC washout indicates a need for time normalization to compare correction efficiency across cells.