An allele of CDC14 that is not inhibited by Net1 bypasses the anaphase arrest caused by a failure in bud growth. (A) NET1-6xHA, NET1-6xHA sec6-4 swe1∆, and NET1-6xHA sec6-4 swe1∆ cdc14TAB6 cells grown overnight in YPD to log phase were released from a G1 phase arrest into fresh media at 25°C. 20 min after release the cultures were shifted to 34°C to inactivate sec6-4 and samples were collected at the indicated time points to assay levels of Clb2. A background band on the Clb2 blots was used as a loading control. (B)NET1-6xHA, NET1-6xHA sec6-4 swe1∆, and NET1-6xHA sec6-4 swe1∆ bfa1∆ cells grown overnight in YPD to log phase were released from a G1 phase arrest into fresh media at 25°C. 20 min after release the cultures were shifted to 34°C to inactivate sec6-4 and samples were collected at the indicated time points to assay levels of Clb2. An anti-Nap1 antibody was used as a loading control. Source data are available for this figure: SourceData F6.