Hyperphosphorylation of Net1 is correlated with the rate and extent of bud growth. (A)NET1∆328-3xHA cells grown overnight to log phase in YPD, YPGal, or YPG/E were released from a G1 phase arrest at 25°C into fresh media containing benomyl. Samples were taken at the indicated times to assay for Net1∆328-3xHA and Clb2. The Net1 blots and the Clb2 blots are from the same samples, so the Net1 blots serve as a loading control for the Clb2 blots. (B) A Coulter Counter was used to analyze cell size as a function of time for the experiments shown in A. Error bars indicate SEM for three biological replicates. Source data are available for this figure: SourceData F4.
Figure 4.

Hyperphosphorylation of Net1 is correlated with the rate and extent of bud growth. (A) NET1 ∆328 -3xHA cells grown overnight to log phase in YPD, YPGal, or YPG/E were released from a G1 phase arrest at 25°C into fresh media containing benomyl. Samples were taken at the indicated times to assay for Net1∆328-3xHA and Clb2. The Net1 blots and the Clb2 blots are from the same samples, so the Net1 blots serve as a loading control for the Clb2 blots. (B) A Coulter Counter was used to analyze cell size as a function of time for the experiments shown in A. Error bars indicate SEM for three biological replicates. Source data are available for this figure: SourceData F4.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal