Figure S8.
Cas13d expression with gRNAs specific for IGS ncRNA reduces ER stress in SETX KO U2OS cells. Activation of the unfolded protein response and ER stress was measured with a CHOP-mCherry reporter (Oh et al., 2012) by FACS with at least 10,000 cells per replicate, three replicates per condition. mCherry fluorescence levels per cell were quantified by FACS in U2OS cells in control cells or SETX KO cells with Cas13d and expression of IGS-specific gRNAs as indicated. Control and SETX KO data are also shown in Fig. 1 L. P values are derived using a two-tailed t test assuming unequal variance, using the mean of the fluorescence values from each biological replicate.** indicates P < 0.005, ** indicates P < 0.0005. Box plots show all measurements from all replicates.

Cas13d expression with gRNAs specific for IGS ncRNA reduces ER stress in SETX KO U2OS cells. Activation of the unfolded protein response and ER stress was measured with a CHOP-mCherry reporter (Oh et al., 2012) by FACS with at least 10,000 cells per replicate, three replicates per condition. mCherry fluorescence levels per cell were quantified by FACS in U2OS cells in control cells or SETX KO cells with Cas13d and expression of IGS-specific gRNAs as indicated. Control and SETX KO data are also shown in Fig. 1 L. P values are derived using a two-tailed t test assuming unequal variance, using the mean of the fluorescence values from each biological replicate.** indicates P < 0.005, ** indicates P < 0.0005. Box plots show all measurements from all replicates.

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