Frequency of tumor clonotypes found in the blood according to assigned cluster and clonotype size in the tumor. The frequency was computed as the number of tumor clonotypes found in the blood (Boolean variable) using bulk RNAseq divided by the total number of clonotypes assigned to a given cluster for each patient for each TCR chain (TCRα [circle] and TCRβ [triangle]). A given clonotype was assigned to the cluster in which the majority of the cells was found. Only clonotypes ≥2 cells were analyzed. The effects of the TCR chain nature (TCRα or TCRβ) and clusters were evaluated using an ANOVA. Tukey’s post-hoc test to identify differences between clusters. The statistical differences are indicated as: *, P < 0.05; ***, P < 0.001 or ns for non-significant, and NA for non-applicable when data points were missing leading to underpower.