Characterization of the Tregs and CD8 ⁺ cells in primary UMs. (A) Representative dot plots of Treg (CD25⁺FoxP3⁺) in CD4⁺ T cells in juxta-tumor and tumor tissues (frequencies in Fig. 1 B). (B) Representative dot plots of CCR8, PD-1, CD39, and ICOS expression in Treg defined as CD4⁺CD25⁺FoxP3⁺ (left) and frequency of Treg expressing these markers in juxta-tumor and tumor tissues (right). (C) Representative dot plots of CD45RA and CCR7 expression defining: naïve: CD45RA⁺CCR7⁺; TEMRA: CD45RA⁺CCR7⁻; effector memory (EM): CD45RA⁻CCR7⁻; central memory (CM): CD45RA⁻CCR7⁺) (left) and frequency of these subsets in CD8⁺ T cells in juxta-tumor and tumor tissues (right). (D) Representative dot plots showing CD8⁺ T cells expressing CD69, CD103, CD27, KLRG1, PD-1, TIM3, LAG3, CD39 in juxta-tumor and tumor (frequencies in Fig. 1 F). (E) Representative dot plots of PD-1 and CD39 expression in CD8⁺ T cells (left) and frequency of CD39⁺PD-1⁺ cells in CD8⁺ T cells from juxta-tumor and tumor tissues (right). (F) Unsupervised analysis of CD3⁺ cells in five juxta-tumor and six tumor tissues. Expression of the indicated markers in the UMAP representation. Coh1 (n = 20) was used in C. Coh2 (n = 7) was used in B. Coh3 (n = 33) was used in D. Coh1 and coh3 were used in E. Coh3 was used in F. Non-parametric Mann–Whitney test was used in B−E. Only P < 0.05 are shown.