H3K36me2 in mouse tissues, E13.5, and preimplantation embryos. (A and B) Immunofluorescence of tissue sections (A) and E13.5 sections (B) stained with antibodies against H3K36me2 (red), H3K9me3 (green), and Hoechst 33342 (blue). Single optical sections of confocal microscope images are shown. (A) Low-power views of merged images are shown on the left (scale bars, 50 μm). Individual and merged images of the indicated area are shown on the right (scale bars, 10 μm). (B) A tiled image covering the whole embryo is shown on the left (scale bar, 500 μm). Single images for the brain (pons area; #1), cartilage primordium (#2), and stomach (#3) (scale bars, 50 μm) and magnified views of the indicated areas are shown from the middle to the right with individual contrast adjustments to compare the localization patterns (scale bars, 10 μm). (C) Different localization of H3K36me2 in maternal PN in zygotes and the two-cell nucleus in preimplantation embryos analyzed by line plots. Intensity profiles of H3K36me2 (red) and DAPI (blue) along the yellow lines are shown. Scale bar, 10 μm.