Figure 5.
NSD2 interacts with SMARCA5 and BAZ1B. (A) Halo-NSD2 or HaloTag alone (Halo) and emGFP-SMARCA5 or emGFP-BAZ1B were co-expressed in HEK293T cells and emGFP-tagged proteins were isolated using anti-GFP nanobody magnetic beads. Cell lysates (input) and 10× concentrated immunoprecipitated materials (IP) were separated on a 7.5% SDS-polyacrylamide gel, transferred to a membrane, and probed with antibodies specific for HaloTag (top) and GFP (bottom). (B) The interaction between Halo-NSD2 mutants and emGFP-SMARCA5 was analyzed by co-IP and immunoblotting as in A. Schematic drawing of NSD2 domains and mutants is shown on top. The positions of size standards and Halo-proteins are indicated. Source data are available for this figure: SourceData F5.

NSD2 interacts with SMARCA5 and BAZ1B. (A) Halo-NSD2 or HaloTag alone (Halo) and emGFP-SMARCA5 or emGFP-BAZ1B were co-expressed in HEK293T cells and emGFP-tagged proteins were isolated using anti-GFP nanobody magnetic beads. Cell lysates (input) and 10× concentrated immunoprecipitated materials (IP) were separated on a 7.5% SDS-polyacrylamide gel, transferred to a membrane, and probed with antibodies specific for HaloTag (top) and GFP (bottom). (B) The interaction between Halo-NSD2 mutants and emGFP-SMARCA5 was analyzed by co-IP and immunoblotting as in A. Schematic drawing of NSD2 domains and mutants is shown on top. The positions of size standards and Halo-proteins are indicated. Source data are available for this figure: SourceData F5.

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