H3K36me2 is enriched in PCH and transposable elements in some mouse cell lines. (A–F) Immunofluorescence. Cells were fixed and stained with antibodies directed against histone modifications and Hoechst 33342. Single optical sections of confocal microscope images are shown with individual contrast adjustments to compare the localization patterns. (A) H3K36me2 (green) and Hoechst 33342 (red) in various mouse cell lines. (B) Localization of H3K36me2 respect to satellite repeats. iMEFs expressing dCas9-EGFP (blue) with sgRNA targeting to major (left) or minor satellite repeats (right) were fixed and stained with fluorescence dye-conjugated antibodies specific for H3K36me2 (green) and H3K9me3 (red). Insets show magnified views of the indicated PCH areas. (C) Pmi28 cells and iMEFs stained with another H3K36me2-specific antibody (green; rabbit monoclonal) with H3K9me3 (red) and Hoechst 33342 (blue). (D–F) Localization of various modifications (blue), such as H3K27me3 (D), H3K9me2 (E), and H3K36me3 (F), with respect to H3K36me2 (green) and H3K9me3 (red) in iMEFs. Insets show magnified views of the indicated PCH areas. (G and H) ChIP-seq signal enrichments of H3K36me2 and H3K9me3 on repeat elements in ESCs and B cells from the TSS to the TES (G) and around TSS (H). Scale bars, 10 μm.