Figure 3.
P2RX7 and microglial TNFα promote daily changes in synaptic GABAAR content and CaMKII phosphorylation. (a) Representative confocal images of Thr286-phosphorylated CaMKII immunoreactivity in L1 show higher intensity at ZT6 than ZT18 in transgenic control mice (tCTL). Scale bars, 20 μm. (b) Mean intensity of Thr286-phosphorylated CaMKII signal in L1 normalized to ZT18 for tCTL, microglia-specific TNFα depletion (micTNFα-KO) and P2rx7-KO mice. n = 37–50 FOVs from four to five mice per group. (c) Mean intensity of GABAARγ2 clusters at gephyrin+VGAT+ synapses normalized to ZT18. n = 48 to 65 FOVs from four to five mice per group. (b and c) *P < 0.05 and **P < 0.01, nested two-tailed t-test. Results are presented as minimum to maximum box plots.

P2RX7 and microglial TNFα promote daily changes in synaptic GABA A R content and CaMKII phosphorylation. (a) Representative confocal images of Thr286-phosphorylated CaMKII immunoreactivity in L1 show higher intensity at ZT6 than ZT18 in transgenic control mice (tCTL). Scale bars, 20 μm. (b) Mean intensity of Thr286-phosphorylated CaMKII signal in L1 normalized to ZT18 for tCTL, microglia-specific TNFα depletion (micTNFα-KO) and P2rx7-KO mice. n = 37–50 FOVs from four to five mice per group. (c) Mean intensity of GABAARγ2 clusters at gephyrin+VGAT+ synapses normalized to ZT18. n = 48 to 65 FOVs from four to five mice per group. (b and c) *P < 0.05 and **P < 0.01, nested two-tailed t-test. Results are presented as minimum to maximum box plots.

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