AMPARs plasticity across the light/dark cycle. (a) Confocal images showing downregulation of GluA2 (cyan) at Homer1⁺ clusters (magenta, arrowheads) at ZT6 in cortical L1. Scale bars, 5 and 1 μm. (b) Left: Mean intensity of GluA2 clusters at Homer1⁺ clusters. No statistical significance by nested two-tailed t test. Right: Cumulative fraction of GluA2 clusters’ intensities at Homer1⁺ clusters in control CTL (black) and PLX3397 treated (green) mice. ****P < 0.0001, Kolmogorov–Smirnoff test. (c) Left: Mean intensity of GluA2 at extrasynaptic sites. No statistical significance by nested two-tailed t test. Right: Cumulative fraction of GluA2 clusters’ intensities at extrasynaptic sites in controls CTL (black) and PLX3397 treated (green) mice. No statistical significance by Kolmogorov–Smirnoff test. (d) Fraction of Homer1⁺ clusters colocalized with GluA2 in controls CTL (black) and PLX3397 treated (green) mice. No statistical significance by nested two-tailed t test. (b–d)n = 46-58 FOVs from four to five mice per group. Results are presented as minimum to maximum box plots and cumulative distributions.