Figure S4.
Septin-bound GFP-CD63 endosomes are mainly acidic. (A) Confocal microscopy image of a live MDCK cell that expresses mCherry-SEPT9_i1 (inverted blue) and GFP-CD63 (inverted forest green) after incubation with LysoView633 (inverted magenta). Outlined regions are shown in higher magnification. Scale bars, 10 and 2 μm (insets). (B) Quantification of the mean percentage (±SEM, error bars) of septin (mCherry-SEPT9_i1) bound GFP-CD63 endosomes with or without LysoView633 in subconfluent MDCK cells (n = 6). Data were statistically analyzed with a Mann-Whitney test. ** P < 0.01. (C) Quantification of the mean percentage (±SEM, error bars) of total GFP-CD63 endosomes with or without LysoView633 in subconfluent MDCK cells (n = 6). Data were statistically analyzed with a Mann-Whitney test. ** P < 0.01.

Septin-bound GFP-CD63 endosomes are mainly acidic. (A) Confocal microscopy image of a live MDCK cell that expresses mCherry-SEPT9_i1 (inverted blue) and GFP-CD63 (inverted forest green) after incubation with LysoView633 (inverted magenta). Outlined regions are shown in higher magnification. Scale bars, 10 and 2 μm (insets). (B) Quantification of the mean percentage (±SEM, error bars) of septin (mCherry-SEPT9_i1) bound GFP-CD63 endosomes with or without LysoView633 in subconfluent MDCK cells (n = 6). Data were statistically analyzed with a Mann-Whitney test. ** P < 0.01. (C) Quantification of the mean percentage (±SEM, error bars) of total GFP-CD63 endosomes with or without LysoView633 in subconfluent MDCK cells (n = 6). Data were statistically analyzed with a Mann-Whitney test. ** P < 0.01.

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