The UBL1 and HVR domains of Nsp3 are required for DMV formation, related to Fig. 4 . (A–I) In cells expressing Nsp4, puncta formed by coexpression of Nsp3 with a deletion of the Mac1 (B), Mac2 (C), Mac3 (D), DPUP (E), UBL2 (F), PL2 (G), NAB (H), or βSM (I) domain show no obvious difference compared with full-length Nsp3 (A). Bars: 5 μm; insets, 2 μm. (J) TEM images of cells coexpressing Nsp3(ΔMac1), Nsp3(ΔMac2), Nsp3(ΔMac3), Nsp3(ΔDPUP), Nsp3(ΔUBL2), Nsp3(ΔPL2), Nsp3(ΔNAB), or Nsp3(ΔβSM) with Nsp4. Bars: 500 nm; insets, 200 nm.