Figure S2.
Conservation, purification, localization, and expression of Faa1. (A) Alphafold model of human FACL4. The distribution of the electrostatic potentials was calculated with the APBS method on the molecular surface of FACL4. Electropositively and electronegatively charged areas are colored in blue and red, respectively. Neutral residues are depicted in white. (B) The size exclusion chromatograms of Faa1-WT-EGFP, Faa1-4D-EGFP, and Faa1-6D-EGFP show that all three proteins elute from the column at the same volume. (C) 3D reconstruction of autophagosomes from different angles. Atg8 (magenta) and Faa1 (cyan). Examples obtained from cells from Fig. 3 E. (D) Normalized protein levels of genomically tagged Faa1-WT, Faa1-4D, and Faa1-6D by Western blot quantification. Data are means ± SD (n = 3). Source data are available for this figure: SourceData FS2.

Conservation, purification, localization, and expression of Faa1. (A) Alphafold model of human FACL4. The distribution of the electrostatic potentials was calculated with the APBS method on the molecular surface of FACL4. Electropositively and electronegatively charged areas are colored in blue and red, respectively. Neutral residues are depicted in white. (B) The size exclusion chromatograms of Faa1-WT-EGFP, Faa1-4D-EGFP, and Faa1-6D-EGFP show that all three proteins elute from the column at the same volume. (C) 3D reconstruction of autophagosomes from different angles. Atg8 (magenta) and Faa1 (cyan). Examples obtained from cells from Fig. 3 E. (D) Normalized protein levels of genomically tagged Faa1-WT, Faa1-4D, and Faa1-6D by Western blot quantification. Data are means ± SD (n = 3). Source data are available for this figure: SourceData FS2.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal