SERPINB5 rescues defects induced by DPM1 loss in primary keratinocytes. (a) Western blot showing expression of the respective SERPIN-GFP constructs in sgDPM1 HaCaT keratinocytes. GFP used as control. GAPDH used as loading control. Panel shows representative of three biological replicates. (b and c) Dispase-based dissociation assays to semiquantitatively assess cell–cell adhesion in primary human keratinocytes overexpressing SERPINB5-GFP in sgDPM1 background (N = 4). One-way ANOVA, Dunnett’s multiple comparison test used for statistics. (d) Images of DSP immunostainings in sgNT1-GFP, sgDPM1-GFP, and sgDPM1-SERPINB5 GFP in primary human keratinocytes. Scale bar: 10 µm distance. Panel represents three biological replicates. (e) Western blot showing SERPINB5 levels in sgNT1 and sgSERPINB5 HaCaT keratinocytes. Image represents three biological replicates. (f and g) Western blot images and quantifications of desmosomal proteins from sgNT1 and sgDPM1 HaCaT keratinocytes expressing the indicated SERPIN-GFP constructs. GAPDH used as internal loading control (N = 3). (h and i) Western blot images and quantifications of DPM1 from sgNT1 and sgSERPINB5 HaCaT keratinocytes. GAPDH used as internal loading control (N = 3). Unpaired Student’s t test. Source data are available for this figure: SourceData FS3.