Figure 5.
Detection of the m6Am site on BICD2 mRNA. (A) The scheme of the single-based resolution m6Am fragments detection strategy by using liquid chromatography-high resolution mass spectrometry (LC-HRMS). (B–E) The primary and secondary MS results of the targeted fragment in BICD2 showing both m6Am or m6A sites detected in the second “A” of this fragment. (F and G) The total ion current (TIC) of the fragment containing m6Am or m6A modification with PCIF1 knockdown.

Detection of the m 6 Am site on BICD2 mRNA. (A) The scheme of the single-based resolution m6Am fragments detection strategy by using liquid chromatography-high resolution mass spectrometry (LC-HRMS). (B–E) The primary and secondary MS results of the targeted fragment in BICD2 showing both m6Am or m6A sites detected in the second “A” of this fragment. (F and G) The total ion current (TIC) of the fragment containing m6Am or m6A modification with PCIF1 knockdown.

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