Figure S2.
The effect of CACNG6, SEMA7A, POMGNT1, WBP4, and IFT27 genes knockdown on ciliation. RPE-1 cells transfected with the indicated siRNAs for 48 h were treated with serum starvation for another 24 h and then subjected to RT-qPCR analysis or immunofluorescence. (A, D, G, J, and M) RT-qPCR analysis of the related mRNAs of the five genes depleted RPE-1 cells. GAPDH is the internal control. (B, E, H, K, and N) Immunofluorescence images of RPE-1 cells with anti-ARL13B (green) and γ-tubulin (red) antibodies. DNA was stained with DAPI (blue). Cilia were indicated by white arrows. Scale bar, 10 μm. (C, F, I, L, and O) Quantification analysis of the percentage of ciliated cells. Data are means ± SD of three independent experiments. Student’s t test was performed. ns, not significant.

The effect of CACNG6, SEMA7A, POMGNT1, WBP4, and IFT27 genes knockdown on ciliation. RPE-1 cells transfected with the indicated siRNAs for 48 h were treated with serum starvation for another 24 h and then subjected to RT-qPCR analysis or immunofluorescence. (A, D, G, J, and M) RT-qPCR analysis of the related mRNAs of the five genes depleted RPE-1 cells. GAPDH is the internal control. (B, E, H, K, and N) Immunofluorescence images of RPE-1 cells with anti-ARL13B (green) and γ-tubulin (red) antibodies. DNA was stained with DAPI (blue). Cilia were indicated by white arrows. Scale bar, 10 μm. (C, F, I, L, and O) Quantification analysis of the percentage of ciliated cells. Data are means ± SD of three independent experiments. Student’s t test was performed. ns, not significant.

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