Macrophage depletion is accompanied by a reduction of T cell infiltration. (A) The densities of CD8 and CD4⁺ Foxp3⁻ effector T cells (CD4 Teffs) were depicted as an absolute number of cells per mg of tumor on day 16 after tumor challenge. Data were pooled from three independent experiments (n = 3 mice per group; one-way ANOVA, post hoc, *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001). (B) The density of CD4⁺ Foxp3⁺ Tregs was depicted as an absolute number of cells per mg of tumor on day 16 after tumor challenge. Data were pooled from three independent experiments (n = 3 mice per group; one-way ANOVA, post hoc, ns, not significant). (C) Quantification of CD8/Treg and CD4⁺ Foxp3⁻ Teff/Treg ratios in day 16 B16-F10 tumors. Data were pooled from three independent experiments (n = 3 mice per group; one-way ANOVA, post hoc ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001). Error bars represent means ± SEM. Irradiated parental B16 tumor cells (Vac) were used as a control for the IVAX group.