Impact of fascin on the growth rates of cofilin clusters. (A) Growth rates of cofilin clusters on single filaments exposed to 200 nM mCherry-cofilin1 in the presence or absence of 200 nM fascin, N = 3 experiments, n > 40 cofilin clusters for each experiment. Large symbols represent averages over individual measures from independent experiments. Paired t test P value = 0.494. (B) Growth rates of cofilin clusters on two-filament bundles exposed to 200 nM cofilin and various fascin concentrations (n = 9, 7, 5 cofilin clusters for 200, 500, and 1,000 nM fascin respectively). One-way ANOVA test P value = 0.56.