Association of Sly1 and its variants with immobilized GST-Sed5. Binding reactions were set up as in Fig. 9 with the indicated Sly1 variants (80 pmol per 500 µl reaction) and GST-Sed5 cytoplasmic domain immobilized on glutathione-agarose (GST-Sed5Cyt; 150 pmol added to beads per reaction). The binding and wash buffer contained 10 mg/ml BSA. After sedimenting and washing the beads, proteins in the pellet were eluted with SDS-PAGE sample buffer, separated on 10% polyacrylamide gels, and stained with Coomassie blue. Source data are available for this figure: SourceData FS5.
Figure S5.

Association of Sly1 and its variants with immobilized GST-Sed5. Binding reactions were set up as in Fig. 9 with the indicated Sly1 variants (80 pmol per 500 µl reaction) and GST-Sed5 cytoplasmic domain immobilized on glutathione-agarose (GST-Sed5Cyt; 150 pmol added to beads per reaction). The binding and wash buffer contained 10 mg/ml BSA. After sedimenting and washing the beads, proteins in the pellet were eluted with SDS-PAGE sample buffer, separated on 10% polyacrylamide gels, and stained with Coomassie blue. Source data are available for this figure: SourceData FS5.

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