Lipid as well as content mixing is defective with Sly1∆loop. Parallel lipid (top) and content mixing (bottom) results are shown from the same sets of reactions. The content mixing traces are identical to those shown in Fig. 5, B and D, and are shown here to facilitate comparison with the lipid mixing data. All reactions contained 3% PEG. (A) The reactions in A omitted Sec17 and Sec18. (B) The reactions in B contained 100 nM each of Sec17 and Sec18, and ATP. Lipid mixing is reported as raw fluorescence counts in arbitrary units. As the membranes mix, FRET from Marina Blue DHPE to NBD-DHPE (initially in separate liposomes) quenches Marina Blue emission at 465 nm. Points and bars in all traces show means ± SEM of data from three separate experiments.
Figure S3.

Lipid as well as content mixing is defective with Sly1∆loop. Parallel lipid (top) and content mixing (bottom) results are shown from the same sets of reactions. The content mixing traces are identical to those shown in Fig. 5, B and D, and are shown here to facilitate comparison with the lipid mixing data. All reactions contained 3% PEG. (A) The reactions in A omitted Sec17 and Sec18. (B) The reactions in B contained 100 nM each of Sec17 and Sec18, and ATP. Lipid mixing is reported as raw fluorescence counts in arbitrary units. As the membranes mix, FRET from Marina Blue DHPE to NBD-DHPE (initially in separate liposomes) quenches Marina Blue emission at 465 nm. Points and bars in all traces show means ± SEM of data from three separate experiments.

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