In silico estimation of membrane binding by Sly1 helix α21 and its polar mutant pα21.(A and B) PMIpred results for helices α21 (A) and pα21 (B) were generated by the server at https://pmipred.fkt.physik.tu-dortmund.de/curvature-sensing/ (van Hilten et al., 2023a, 2023b, Preprint). Similar results for α21 and pα21 were obtained when the model was initialized with either negatively charged or neutral membranes. These estimated binding parameters for α21 predict stronger binding than for the well-characterized GMAP-210 ALPS domain, and similar binding to the engineered high-affinity ALPScond mutant (Magdeleine et al., 2016; van Hilten et al., 2023a).
Figure S2.

In silico estimation of membrane binding by Sly1 helix α21 and its polar mutant pα21. (A and B) PMIpred results for helices α21 (A) and pα21 (B) were generated by the server at https://pmipred.fkt.physik.tu-dortmund.de/curvature-sensing/ (van Hilten et al., 2023a, 2023b, Preprint). Similar results for α21 and pα21 were obtained when the model was initialized with either negatively charged or neutral membranes. These estimated binding parameters for α21 predict stronger binding than for the well-characterized GMAP-210 ALPS domain, and similar binding to the engineered high-affinity ALPScond mutant (Magdeleine et al., 2016; van Hilten et al., 2023a).

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