Develop inhibitory peptides to antagonize the WW-NLS in YAP1 nuclear import. (A) The pull-down experiment shows that the CPY6 peptide blocks the interaction of FLAG-tagged KPNA2 with Fc-fused YAP1 in HEK293T cells. (B) The WW domains from different proteins are pulled down by the CPY6 peptide fused to Fc fragment of IgG in HEK293T cells. TCL, total cell lysates. (C) Representative immunostaining images show the subcellular localization of YAP1 in AsPC1 cells, and representative western blotting shows the depletion of YAP1 expression in YAP1-KD AsPC1 cells. (D) Representative western blotting shows the depletion of YAP1 expression in YAP1-KD MDA-MB-231 cells. (E) Representative images show spheroids of AsPC1 cells following 1-wk culture with 1 μM of synthesized PPPY peptide. Source data are available for this figure: SourceData FS5.