The WW-NLS bypasses the mediation of cNLS. (A) The schematic diagram illustrates the potential hitchhike mechanism for YAP1 nuclear import, and the structure (PDB: 3UKX) illustrates the competitive binding of Bimax2 to KPNA2. (B) Representative images show the subcellular localization of 2xcNLS−3xGFP and tdWWs (YAP1)−3xGFP upon ectopic expression of Bimax2 in HEK293T cells, and the scatter chart shows their nucleocytoplasmic ratios (representative of three independent experiments, n represents the total number of the measured cells, two-tailed ANOVA test). (C) Representative images show LMB-induced subcellular localization of YAP1-GFP upon ectopic expression of Bimax2 in HEK293T cells, and the scatter chart shows their nucleocytoplasmic ratios (representative of three independent experiments, n represents the total number of the measured cells, two-tailed ANOVA test). LMB, 0.5 μM, 2 h. (D) Representative images show the subcellular localization of cNLS-YAP1-ΔWWs-GFP upon ectopic expression of Bimax2 in HEK293T cells, and the scatter chart shows their nucleocytoplasmic ratios (representative of three independent experiments, n represents the total number of the measured cells, two-tailed ANOVA test).