S2814A and S2814D; NND and CDF with and without Iso . (A and B) NNDs of RyR2 were acquired from S2814A (A) and S2814D (B) mice hearts, (i) saline-perfused and (ii) treated with ISO. CDF of tetramer NND acquired from (A iii) S2814A and (B iii) S2814D hearts perfused with saline or Iso compared to their WT.