Regulation of IMM diffusion barriers. (A–C) Dual-color FLIP in fis1∆ cells expressing Tom20-GFP and matrix-mCherry. Quantification of Tom20-GFP FLIP in fis1∆ (A; n = 30 cells), fis1∆ bud6∆ (B; n = 23 cells), fis1∆ shs1∆ (C; n = 30 cells) cells. Tom20-GFP bleaching curves from A are overlayed in B and C as gray lines. (D–G) Dual-color FLIP in fis1∆ cells expressing Yta12-GFP and matrix-mCherry. Quantification of Yta12-GFP FLIP in fis1∆ (D; n = 36 cells), fis1∆ sur2∆ (E; n = 21 cells), fis1∆ crd1∆ (F; n = 20 cells), and fis1∆ mic60∆ (G; n = 25 cells). Yta12-GFP bleaching curves from D are overlayed in E–G as gray lines. Shadows represent mean ± SE.