Other IMM and OMM proteins. (A–E) Single-color FLIP in fis1∆ cells expressing GFP-tagged mitochondrial IMM (A–C) or OMM (D and E) proteins in the absence of matrix-mCherry. Representative images and quantification of Oxa1-GFP (A; IMM, n = 10 cells), Yme1-GFP (B; IMM, n = 12 cells), Atm1-GFP (C; IMM, n = 10 cells), Om45-GFP (D; OMM, n = 12 cells), and Por1-GFP (E; OMM, n = 11 cells). Photobleach was applied in the GFP channel as indicated by white circles. Shadows represent mean ± SD. Images are a sum projection of five z-stacks taken at 0.5-μm intervals. Scale bar: 3 μm.