Overview of functional clusters from image-based profiling of secondary screen data. Displayed is the phenotypic feature heatmap generated by hierarchical clustering. The data, including gene names and feature titles, can be explored by zooming in within Data S1 as well as in Table S8. The scale of rZ values (central reference = NTC) was adjusted based on the minimum and maximum values of individual features. Labels to the right highlight a subset of functional clusters, as well as the positions of the NTC (neutral control) crRNAs and crRNAs targeting CAPZB and DYNC1LI1. “Cytoplasm” refers to features associated with the background Hoechst staining in the cytoplasm.