Elm1 stabilizes Gin4 at the bud neck prior to cytokinesis. (A) Maximum-intensity projection images of representative YEF10559 (elm1∆ GIN4-GFP CDC3-mCherry mRuby2-TUB1) cells from a time-lapse series taken with a 2-min interval with Gin4-GFP in green at indicated times. T = 0 is mitotic spindle break. Gray dashed line is the cell periphery. Yellow boxed region is the area used for measurements in B and D and to make montages for C. Scale bar = 2 μm. (B) Quantification of Gin4-GFP signal from cells in A and C. Shown is integrated measured background subtracted intensity of Gin4-GFP in WT (dark green) and elm1∆ (light green) cells from the sum projection of given number cells for each strain. The mean is plotted with error bars being the SD. a.u. = arbitrary units. (C) Montages of representative YEF10558 (GIN4-GFP CDC3-mCherry mRuby2-TUB1) and YEF10559 (elm1∆ Gin4-GFP Cdc3-mCherry mRuby2-TUB1) cells showing maximum-intensity projections from 20 min before to 10 min after mitotic spindle break from time-lapse series taken with a 2-min interval. T = 0 is mitotic spindle break. Scale bars = 1 μm. (D) Quantification of Gin4-GFP signal from cells in A and C. Shown is background subtracted intensity relative to the maximum value measured of Gin4-GFP in WT (dark green) and elm1∆ (light green) cells from the sum projection of given number cells for each strain. The mean is plotted with error bars being the SD.