Gin4 retains septins at the bud cortex in elm1∆ cells. (A) Representative images of YEF10238 (elm1∆ gin4∆ CDC3-GFP mRuby2-TUB1) cells with brightfield (left) and maximum-intensity projection of merged Cdc3-GFP in green and mRuby2-Tub1 in magenta (right). Scale bars = 5 μm. (B) Maximum-intensity projection images of representative elm1∆ gin4∆ (YEF10238), elm1∆ gin4KD (YEF11144), and elm1∆ gin4∆KA1 (YEF10990) cells from a time-lapse series taken with a 2-min interval with Cdc3-GFP in green and mRuby2-Tub1 in magenta at indicated times. T = 0 is bud emergence. Gray dashed line is the cell periphery. Yellow boxed region is the area used for measurements in C–E. Scale bars = 2 μm. (C) Quantification of cells in Fig. 3 B and B. Shown is background subtracted intensity of Cdc3-GFP in WT (green), elm1∆ (gray), gin4∆ (yellow), and elm1∆ gin4∆ (blue) relative to the maximum value measured from the sum projection of the given number of cells for each strain. Curves for WT, elm1∆, and gin4∆ are the same used in Fig. 3 C and are at 30% opacity for ease of comparing the kinetic signature of elm1∆ gin4∆ cells to WT and each single deletion mutant. The mean is plotted with error bars being the SD. (D) Quantification of cells in Fig. 3 B and B. Shown is the background subtracted intensity of Cdc3-GFP in WT (green), elm1∆ (gray), gin4∆ (yellow), elm1∆ gin4∆ (blue), and elm1∆ gin4KD (black) relative to the maximum value measured from the sum projection of given number cells for each strain. Curves for WT, elm1∆, and gin4∆ are the same used in Fig. 3 C and that of elm1∆ gin4∆ is the same as used in C and are at 30% opacity for ease of comparing the kinetic signature of elm1∆ gin4KD cells to previously analyzed cells. The mean is plotted with error bars being the SD. (E) Representative image of YEF10989 (elm1∆ gin4KD-GFP CDC3-mCherry) cells with brightfield (left) and maximum intensity projection of Gin4KD-GFP in green (middle) and Cdc3-mCherry in magenta (right). Gray dashed line is cell periphery. Scale bar = 5 μm. (F) Quantification of cells in Fig. 3 B and B. Shown is the background subtracted intensity of Cdc3-GFP in WT (green), elm1∆ (gray), gin4∆ (yellow), elm1∆ gin4∆ (blue), and elm1∆ gin4∆KA1 (black) relative to the maximum value measured from the sum projection of given number cells for each strain. Curves for WT, elm1∆, and gin4∆ are the same used in Fig. 3 C and that of elm1∆ gin4∆ is the same as used in C and are at 30% opacity for ease of comparing the kinetic signature of elm1∆ gin4∆KA1 cells to previously analyzed cells. The mean is plotted with error bars being the SD.
Figure 8.

Gin4 retains septins at the bud cortex in elm1∆ cells. (A) Representative images of YEF10238 (elm1∆ gin4∆ CDC3-GFP mRuby2-TUB1) cells with brightfield (left) and maximum-intensity projection of merged Cdc3-GFP in green and mRuby2-Tub1 in magenta (right). Scale bars = 5 μm. (B) Maximum-intensity projection images of representative elm1∆ gin4∆ (YEF10238), elm1∆ gin4KD (YEF11144), and elm1∆ gin4∆KA1 (YEF10990) cells from a time-lapse series taken with a 2-min interval with Cdc3-GFP in green and mRuby2-Tub1 in magenta at indicated times. T = 0 is bud emergence. Gray dashed line is the cell periphery. Yellow boxed region is the area used for measurements in C–E. Scale bars = 2 μm. (C) Quantification of cells in Fig. 3 B and B. Shown is background subtracted intensity of Cdc3-GFP in WT (green), elm1∆ (gray), gin4∆ (yellow), and elm1∆ gin4∆ (blue) relative to the maximum value measured from the sum projection of the given number of cells for each strain. Curves for WT, elm1∆, and gin4∆ are the same used in Fig. 3 C and are at 30% opacity for ease of comparing the kinetic signature of elm1∆ gin4∆ cells to WT and each single deletion mutant. The mean is plotted with error bars being the SD. (D) Quantification of cells in Fig. 3 B and B. Shown is the background subtracted intensity of Cdc3-GFP in WT (green), elm1∆ (gray), gin4∆ (yellow), elm1∆ gin4∆ (blue), and elm1∆ gin4KD (black) relative to the maximum value measured from the sum projection of given number cells for each strain. Curves for WT, elm1∆, and gin4∆ are the same used in Fig. 3 C and that of elm1∆ gin4∆ is the same as used in C and are at 30% opacity for ease of comparing the kinetic signature of elm1∆ gin4KD cells to previously analyzed cells. The mean is plotted with error bars being the SD. (E) Representative image of YEF10989 (elm1∆ gin4KD-GFP CDC3-mCherry) cells with brightfield (left) and maximum intensity projection of Gin4KD-GFP in green (middle) and Cdc3-mCherry in magenta (right). Gray dashed line is cell periphery. Scale bar = 5 μm. (F) Quantification of cells in Fig. 3 B and B. Shown is the background subtracted intensity of Cdc3-GFP in WT (green), elm1∆ (gray), gin4∆ (yellow), elm1∆ gin4∆ (blue), and elm1∆ gin4∆KA1 (black) relative to the maximum value measured from the sum projection of given number cells for each strain. Curves for WT, elm1∆, and gin4∆ are the same used in Fig. 3 C and that of elm1∆ gin4∆ is the same as used in C and are at 30% opacity for ease of comparing the kinetic signature of elm1∆ gin4∆KA1 cells to previously analyzed cells. The mean is plotted with error bars being the SD.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal