Artificial tethering of Elm1 to bud neck can rescue gin4∆ phenotypes. (A) Representative images of indicated cells grown overnight at either 25°C (left) or 37°C (right) with differential interference contrast (DIC) and maximum-intensity projections of Shs1-GFP in green and Elm1-mApple-GBP in magenta. Strains used are as follows from top to bottom: YEF9448 (ELM1-mApple-GBP), YEF10665 (SHS1-GFP ELM1-mApple-GBP), YEF9485 (gin4∆ ELM1-mApple-GBP), and YEF10666 (gin4∆ SHS1-GFP ELM1-mApple-GBP). Gray dashed line indicates the cell periphery. Scale bars = 5 μm. (B) Quantification of the ratio of round cells in strains used in A. Plotted is the average of three independent experiments of n ≥ 100 cells. Error bars are SD. Asterisks (*) indicate significantly different sample sets by unpaired Student’s t test (P < 0.05). (C) Representative images of indicated cells grown overnight at 25°C with differential interference contrast (DIC) and maximum intensity projections of Shs1-GFP in green and Elm1^KD-mApple-GBP in magenta. Strains used are as follows from top to bottom: YEF9335 (elm1^KD-mApple-GBP), YEF9362 (SHS1-GFP elm1^KD-mApple-GBP), YEF10738 (gin4∆ elm1^KD-mApple-GBP), and YEF10743 (gin4∆ SHS1-GFP elm1^KD-mApple-GBP). Gray dashed line indicates the cell periphery. Scale bars = 5 μm. (D) Quantification of the ratio of round cells in strains used in A (Elm1^WT) and C (Elm1^KD). Plotted is the average of three independent experiments of n ≥ 100 cells. Error bars are SD. Asterisks (*) indicate significantly different sample sets by unpaired Student’s t test (P < 0.05).