Deletion of BNI5 does not exacerbate the cell growth and morphologies of Elm1 phosphorylation mutants. (A) The sensitivity of bni5∆ cells with elm1 mutants was tested on SC plates. 10-fold serial dilutions of YEF11761 (bni5∆ GFP-ELM1^WTCDC3-mCherry), YEF11762 (bni5∆ GFP-elm1^S519ACDC3-mCherry), YEF11763 (bni5∆ GFP-elm1^S519DCDC3-mCherry), YEF11764 (bni5∆ GFP-elm1^7ACDC3-mCherry), and YEF11765 (bni5∆ GFP-elm1^7DCDC3-mCherry) cultures were spotted on SC plates and incubated at 25°C (left) and 37°C (right) for 3 d. (B and C) Representative images of the indicated bni5∆ cells were cultured at 25°C (B) and 37°C (C) overnight with brightfield (top) and maximum-intensity projections of GFP-tagged Elm1 mutants shown in green (middle) and Cdc3-mCherry shown in magenta (bottom). Strains used are listed in A. The dashed line indicates the cell periphery. Scale bars = 2 µm.