Gin4 is displaced from the bud neck concurrently with septins in elm1∆ cells. (A) Maximum-intensity projection images of representative YEF10559 cells (elm1∆ GIN4-GFP CDC3-mCherry mRuby2-TUB1) from a time-lapse series taken with a 2-min interval with Gin4-GFP in green (left), Cdc3-mCherry and mRuby2-Tub1 (tubulin) in magenta (center), and merged (right) at indicated times. T = 0 is bud emergence, gray dashed line is the cell periphery, yellow boxed region is the zoomed-in area used to create montages for C, scale bars = 2 μm. (B) Quantification of cells in A and YEF10558 (GIN4-GFP CDC3-mCherry mRuby2-TUB1). Shown is the background subtracted intensity of Gin4-GFP in dark green (WT) or light green (elm1∆) and Cdc3-mCherry in magenta (WT) or tan (elm1∆) relative to the maximum value measured from the sum projection of a given number of cells for each strain. The mean is plotted with error bars being the SD. (C) Montages of Gin4-GFP in representative WT (YEF10558), elm1∆ (YEF10559), and shs1∆ (YEF11454) cells showing maximum-intensity projections from 12 min before to 40 min after bud emergence from time-lapse series taken with a 2-min interval. T = 0 is bud emergence, scale bars = 1 μm. (D) Quantification of cells in C. Shown is the background subtracted intensity of Gin4-GFP in WT (green), elm1∆ (gray), and shs1∆ (yellow) relative to the maximum value measured from the sum projection of the given number of cells for each strain. WT and elm1∆ curves are the same values used for B. The mean is plotted with error bars being the SD. (E) Quantification of cells in C. Shown is the integrated measured background subtracted intensity of Gin4-GFP in WT (green), elm1∆ (gray), and shs1∆ (yellow) from the sum projection of the given number of cells for each strain. The mean is plotted with error bars being the SD. a.u. = arbitrary units.
Figure 2.

Gin4 is displaced from the bud neck concurrently with septins in elm1∆ cells. (A) Maximum-intensity projection images of representative YEF10559 cells (elm1∆ GIN4-GFP CDC3-mCherry mRuby2-TUB1) from a time-lapse series taken with a 2-min interval with Gin4-GFP in green (left), Cdc3-mCherry and mRuby2-Tub1 (tubulin) in magenta (center), and merged (right) at indicated times. T = 0 is bud emergence, gray dashed line is the cell periphery, yellow boxed region is the zoomed-in area used to create montages for C, scale bars = 2 μm. (B) Quantification of cells in A and YEF10558 (GIN4-GFP CDC3-mCherry mRuby2-TUB1). Shown is the background subtracted intensity of Gin4-GFP in dark green (WT) or light green (elm1∆) and Cdc3-mCherry in magenta (WT) or tan (elm1∆) relative to the maximum value measured from the sum projection of a given number of cells for each strain. The mean is plotted with error bars being the SD. (C) Montages of Gin4-GFP in representative WT (YEF10558), elm1∆ (YEF10559), and shs1∆ (YEF11454) cells showing maximum-intensity projections from 12 min before to 40 min after bud emergence from time-lapse series taken with a 2-min interval. T = 0 is bud emergence, scale bars = 1 μm. (D) Quantification of cells in C. Shown is the background subtracted intensity of Gin4-GFP in WT (green), elm1∆ (gray), and shs1∆ (yellow) relative to the maximum value measured from the sum projection of the given number of cells for each strain. WT and elm1∆ curves are the same values used for B. The mean is plotted with error bars being the SD. (E) Quantification of cells in C. Shown is the integrated measured background subtracted intensity of Gin4-GFP in WT (green), elm1∆ (gray), and shs1∆ (yellow) from the sum projection of the given number of cells for each strain. The mean is plotted with error bars being the SD. a.u. = arbitrary units.

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