Gin4 localizes to the bud neck prior to Elm1 during bud emergence and maintains localization after Elm1 prior to cytokinesis. (A) Montages of representative YEF10802 cells showing maximum-intensity projections of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta from 12 min before to 40 min after bud emergence from time-lapse series taken with a 2-min interval. T = 0 is bud emergence. Scale bars = 1 μm. (B) Quantification of cells in A. Shown is the background subtracted intensity of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta relative to the maximum value measured from the sum projection of the given number of cells. The mean is plotted with error bars being the SD. (C) Montages of representative YEF10802 cells showing maximum-intensity projections of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta from 16 min before to 10 min after mitotic spindle break from time-lapse series taken with a 2-min interval. T = 0 is mitotic spindle break. Scale bars = 1 μm. (D) Quantification of cells in C. Shown is the background subtracted intensity of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta relative to the maximum value measured from the sum projection of the given number of cells. The mean is plotted with error bars being the SD.
Figure S1.

Gin4 localizes to the bud neck prior to Elm1 during bud emergence and maintains localization after Elm1 prior to cytokinesis. (A) Montages of representative YEF10802 cells showing maximum-intensity projections of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta from 12 min before to 40 min after bud emergence from time-lapse series taken with a 2-min interval. T = 0 is bud emergence. Scale bars = 1 μm. (B) Quantification of cells in A. Shown is the background subtracted intensity of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta relative to the maximum value measured from the sum projection of the given number of cells. The mean is plotted with error bars being the SD. (C) Montages of representative YEF10802 cells showing maximum-intensity projections of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta from 16 min before to 10 min after mitotic spindle break from time-lapse series taken with a 2-min interval. T = 0 is mitotic spindle break. Scale bars = 1 μm. (D) Quantification of cells in C. Shown is the background subtracted intensity of Elm1-GFP in green and Gin4-mScarlet mRuby2-Tub1 in magenta relative to the maximum value measured from the sum projection of the given number of cells. The mean is plotted with error bars being the SD.

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