The spatiotemporal dynamics of Elm1 and Gin4 localization during the cell cycle. (A) Protein maps of Elm1 (left) and Gin4 (right) with relevant domains labeled. Numbers indicate amino acids of labeled domain boundaries. (B) Top: Maximum-intensity projection images of representative WT cells with either Elm1-GFP (left, strain YEF9305) or Gin4-GFP (right, strain YEF10441) in green and Cdc3-mCherry (septin) in magenta at the nascent septin ring and septin hourglass stages. Gray dashed line is the cell periphery, yellow boxed region is the zoomed-in area used to create montages on the bottom, scale bar = 2 μm. Bottom: Montages of yellow boxed region in cells shown in the top showing maximum-intensity projections of Elm1-GFP or Gin4-GFP in green and Cdc3-mCherry in magenta from 12 min before to 40 min after bud emergence with selected frames from time-lapse series taken with a 2-min interval. For this and all subsequent montages, the mother (M) side is to the left and the daughter (D) side is to the right. T = 0 is bud emergence unless indicated otherwise. Scale bars = 1 μm. (C) Quantification of cells in B. Shown is the background subtracted intensity of Elm1-GFP (left) or Gin4-GFP (right) in green and Cdc3-mCherry in magenta relative to the maximum value measured from the sum projection of the given number of cells for each strain. The mean is plotted with error bars being the SD. (D) Montages of representative WT cells showing maximum-intensity projections of Elm1-GFP (left, strain YEF9305) or Gin4-GFP (right, YEF10441) in green and Cdc3-mCherry in magenta from 16 min before to 10 min after septin HDR from time-lapse series taken with a 2-min interval. T = 0 is septin HDR as indicated by septin intensity drop. Yellow box indicates the septin double ring. Scale bars = 1 μm. (E) Quantification of cells in D. Shown is the background subtracted intensity of Elm1-GFP (left) or Gin4-GFP (right) in green and Cdc3-mCherry in magenta relative to the maximum value measured from the sum projection of a given number of cells for each strain. The mean is plotted with error bars being the SD.
Figure 1.

The spatiotemporal dynamics of Elm1 and Gin4 localization during the cell cycle. (A) Protein maps of Elm1 (left) and Gin4 (right) with relevant domains labeled. Numbers indicate amino acids of labeled domain boundaries. (B) Top: Maximum-intensity projection images of representative WT cells with either Elm1-GFP (left, strain YEF9305) or Gin4-GFP (right, strain YEF10441) in green and Cdc3-mCherry (septin) in magenta at the nascent septin ring and septin hourglass stages. Gray dashed line is the cell periphery, yellow boxed region is the zoomed-in area used to create montages on the bottom, scale bar = 2 μm. Bottom: Montages of yellow boxed region in cells shown in the top showing maximum-intensity projections of Elm1-GFP or Gin4-GFP in green and Cdc3-mCherry in magenta from 12 min before to 40 min after bud emergence with selected frames from time-lapse series taken with a 2-min interval. For this and all subsequent montages, the mother (M) side is to the left and the daughter (D) side is to the right. T = 0 is bud emergence unless indicated otherwise. Scale bars = 1 μm. (C) Quantification of cells in B. Shown is the background subtracted intensity of Elm1-GFP (left) or Gin4-GFP (right) in green and Cdc3-mCherry in magenta relative to the maximum value measured from the sum projection of the given number of cells for each strain. The mean is plotted with error bars being the SD. (D) Montages of representative WT cells showing maximum-intensity projections of Elm1-GFP (left, strain YEF9305) or Gin4-GFP (right, YEF10441) in green and Cdc3-mCherry in magenta from 16 min before to 10 min after septin HDR from time-lapse series taken with a 2-min interval. T = 0 is septin HDR as indicated by septin intensity drop. Yellow box indicates the septin double ring. Scale bars = 1 μm. (E) Quantification of cells in D. Shown is the background subtracted intensity of Elm1-GFP (left) or Gin4-GFP (right) in green and Cdc3-mCherry in magenta relative to the maximum value measured from the sum projection of a given number of cells for each strain. The mean is plotted with error bars being the SD.

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