Crystal structure of apo and ligand-binding FABP7. (A–C) The 2Fo-Fc electron density map showing FABP7 with water and cholesterol and 25-HC as the ligand. (D) Detailed view of FABP7 interacting with 25-HC (light orange sticks). Hydrogen bonds formed between FABP7 and the hydroxyl group of 25-HC are denoted by red dashed lines. The hydrophobic interactions between FABP7 F58 and 25-HC are shown by black dashed lines. Key residues for 25-HC binding are indicated.