FABP7 mediates the egress of low-density lipoprotein (LDL)-derived cholesterol from lysosomes. (A–C) WT and two lines of FABP7 KO cells (FABP7 KO1 and FABP7 KO2) were incubated in normal medium (10% fetal bovine serum, A), lipoprotein-deficient medium (5% lipoprotein-deficient serum, B), or endogenous cholesterol-deficient medium (10% fetal bovine serum with 1 µM lovastatin and 10 µM mevalonate, C) as indicated for 16 h. Cells were fixed and stained with filipin (magenta) and the anti-LAMP1 antibody (green). Boxed areas are shown at a higher magnification on the right. Scale bars, 10 µm (main), 1 µm (inset). (D) Quantification of relative fluorescence intensity of filipin in A–C. Data are presented as median with interquartile range (n = 418 cells from three independent experiments). Mann–Whitney U test. Compared with WT cells grown under the same conditions. (E and F) WT and two lines of FABP7 KO cells (FABP7 KO1 and FABP7 KO2) were incubated in lipoprotein-deficient medium (E) or lipoprotein-deficient medium supplemented with 30 µg/ml LDL (F) for 16 h and stained with filipin (magenta) and the anti-LAMP1 antibody (green). Boxed areas are shown at a higher magnification on the right. Scale bars, 10 µm (main), 1 µm (inset). (G) Quantification of relative fluorescence intensity of filipin in E and F. Data are presented as median with interquartile range (n = 295 cells from three independent experiments). Mann–Whitney U test. Compared with WT cells grown under the same conditions.