![Cdh1 KO promotes KP-driven gastric tumorigenesis. (A) Bright-field images of KP and EKP cells in low and high magnification. Scale bars: 100 μm (upper panels); 50 μm (lower panels). (B) Crystal violet staining of KP and EKP GOs-derived cells. (C) Bright-field images of KP and EKP allograft tumors; tumor incidence of allograft tumors. (D and E) Plot for tumor mass (D) and tumor size (E) assessment of KP and EKP allografts. (F) H&E staining of KP and EKP allograft tumors (n ≥ 3). Scale bars (from left to right): 50, 200, 50 μm; Md: middle; Ct: cortex. (G and H) Immunostaining of KP and EKP allograft tumors (n ≥ 3) for MKI67 (G) and E-cadherin (H). Scale bars: 50 μm. (I) Statistics analysis of MKi67 staining in Fig. 4 G. P values were calculated using Student’s t test; error bars: SD. (J–O) CD3 (J), CD4 (K), CD8 (L), PDCD1 (M), TIM3 (N) staining and CD11B/LY6G co-staining (O) of KP and EKP allograft tumors (n ≥ 3). Left panels (low magnification [low mag]; right panels (high magnification [high mag]). Scale bars: 50 μm (low mag) and 20 μm (high mag). (P–U) Statistics analysis of CD3 (P), CD4 (Q), CD8 (R), PDCD1 (S), TIM3 (T) staining and CD11B/LY6G co-staining (U). The positive cell percentage indicates the area of cells expressing a specific marker divided by the total field–occupied cells stained by DAPI in the same area, which allows for normalization. Md: middle; Ct: cortex. P values were calculated using the one-way ANOVA; error bars: SD. ns: P > 0.05; *: P ≤ 0.05; **: P ≤ 0.01; ***: P ≤ 0.001. All data are derived from two or more independent experiments with the indicated number of mice.](https://cdn.rupress.org/rup/content_public/journal/jem/221/4/10.1084_jem.20230561/1/jem_20230561_fig4.png?Expires=1767699137&Signature=dPZllhijPGVgedhTJolyoLN7SBPCrqMPe1m9-rIKJkLIefhOqOZzk4dx1szv94MkZ6BwGNlJcz7UWC1jjx2JJvkY8d6rySFS3fm-l0-6-ZDQ0EP0VrisjbVoXxTfrdxgOmiMES6NYB496wyRGk3qrStSwJh8Whvv5RtuekGr9ja1Ss3WMmBg35QJm-4tGzzikZ5b~O2DKY~G9w2S44KWvW0-B-cqWusnM4EjF-WPxFCs9rTPxEbPFTsYwTFAGU~H3jpZMdZAc-PhJEzDQNCZjFyLf9Zg8bPKgStlRDVbHAb0-Y-eGbNJRQRFUlOooDzawA29Av~gVLusX0ymJIu46g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cdh1 KO promotes KP-driven gastric tumorigenesis. (A) Bright-field images of KP and EKP cells in low and high magnification. Scale bars: 100 μm (upper panels); 50 μm (lower panels). (B) Crystal violet staining of KP and EKP GOs-derived cells. (C) Bright-field images of KP and EKP allograft tumors; tumor incidence of allograft tumors. (D and E) Plot for tumor mass (D) and tumor size (E) assessment of KP and EKP allografts. (F) H&E staining of KP and EKP allograft tumors (n ≥ 3). Scale bars (from left to right): 50, 200, 50 μm; Md: middle; Ct: cortex. (G and H) Immunostaining of KP and EKP allograft tumors (n ≥ 3) for MKI67 (G) and E-cadherin (H). Scale bars: 50 μm. (I) Statistics analysis of MKi67 staining in Fig. 4 G. P values were calculated using Student’s t test; error bars: SD. (J–O) CD3 (J), CD4 (K), CD8 (L), PDCD1 (M), TIM3 (N) staining and CD11B/LY6G co-staining (O) of KP and EKP allograft tumors (n ≥ 3). Left panels (low magnification [low mag]; right panels (high magnification [high mag]). Scale bars: 50 μm (low mag) and 20 μm (high mag). (P–U) Statistics analysis of CD3 (P), CD4 (Q), CD8 (R), PDCD1 (S), TIM3 (T) staining and CD11B/LY6G co-staining (U). The positive cell percentage indicates the area of cells expressing a specific marker divided by the total field–occupied cells stained by DAPI in the same area, which allows for normalization. Md: middle; Ct: cortex. P values were calculated using the one-way ANOVA; error bars: SD. ns: P > 0.05; *: P ≤ 0.05; **: P ≤ 0.01; ***: P ≤ 0.001. All data are derived from two or more independent experiments with the indicated number of mice.