Figure 2.
VPS13C deficiency disrupts inter-lysosomal contacts in hiPSC-derived dopaminergic neurons. (A) Time-lapse images of L-L contacts showing tethered lysosomes (yellow arrows) that untether at 42 and 29 s, respectively (white arrows), in control conditions but remain tethered in VPS13C KD neurons until 129 and 147 s, respectively. The majority of lysosomes in VPS13C KD neurons stay in contact until 180 s. Scale bar: 1 µm. (B) Quantification of the percentage of lysosomes in stable L-L contacts (≥10 s). (C and D) Quantification and histogram distribution of the minimum duration of L-L contacts. (N = 4, from n = 12 cells). (E and F) Representative 3D confocal images of L-L contacts in control neurons (E) and VPS13C KD neurons (F). Yellow arrows indicate the point of contact between two lysosomes in xy, yz, and xz projection (scale bar: 0.5 µm). Data represented as mean ± SEM; unpaired two-tailed t test (B and C); ****P < 0.0001 (B and C).

VPS13C deficiency disrupts inter-lysosomal contacts in hiPSC-derived dopaminergic neurons. (A) Time-lapse images of L-L contacts showing tethered lysosomes (yellow arrows) that untether at 42 and 29 s, respectively (white arrows), in control conditions but remain tethered in VPS13C KD neurons until 129 and 147 s, respectively. The majority of lysosomes in VPS13C KD neurons stay in contact until 180 s. Scale bar: 1 µm. (B) Quantification of the percentage of lysosomes in stable L-L contacts (≥10 s). (C and D) Quantification and histogram distribution of the minimum duration of L-L contacts. (N = 4, from n = 12 cells). (E and F) Representative 3D confocal images of L-L contacts in control neurons (E) and VPS13C KD neurons (F). Yellow arrows indicate the point of contact between two lysosomes in xy, yz, and xz projection (scale bar: 0.5 µm). Data represented as mean ± SEM; unpaired two-tailed t test (B and C); ****P < 0.0001 (B and C).

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