Molecular counting workflow. (A) Depiction of the filter used to identify candles unsuitable for analysis (i.e., too close to another candle or partially out of Z-range). (B) Example of standard candle image before and after filter. (C) Example image of candle deemed passable by the filter in XY and ZY. (D) Depiction of NM2 filament identification workflow with (1) lamella isolation, (2) filament identification by puncta ∼300 nm apart with proof of principle NM2-tail tag to confirm filament, and (3) examples of the 2-puncta, 3-puncta, and 4-puncta identifications.