Leading edge retractions with EGFP-VASP. Scarlet-NM2A cells transiently expressing mEGFP-VASP imaged with confocal microscopy every second. The image is a sum intensity projection of a z-stack with NM2 in blue and VASP in inverted grayscale. Scale bar = 10 μm. The red line indicates the ROI used for subsequent kymograph in the right panel. Gray dotted lines connect leading edge retractions with NM2 filament appearance.