Pharmacological blocking TRPV channels interrupts cytoskeleton mobilization. (A) Representative TIRFM images of F-actin and BCR location. Cells were stimulated with PLB antigen-presenting system in the absence or presence of RR (10 μM). Scale bars, 1.5 μm. (B) Cellular colocalization of TRPV2 and F-actin with or without RR (n > 40). Quantification of PCI between TRPV2 and F-actin. (C) Quantification of MFI of F-actin accumulation in immunological synapse at the indicated time points (n > 40). Cells were stimulated with PLB antigen-presenting system in the absence or presence of RR (10 μM). Unpaired two-tailed t test in B and C. ns, not significant, ***P < 0.001. Data are mean ± SEM. Data are representative of three independent experiments.