PC4-OV promotes liver cancer cell growth in vitro and in vivo. Relative to Fig. 5. (A) Western blot and qPCR showing PC4 expression in Huh7 and HepG2 cells stably overexpressing control or PC4. Data are generated from n = 3 biological replicates. (B) Cell viability analysis by CCK8 assay in Huh7 cells and HepG2 cells with control or PC4 stable overexpression. Data are generated from n = 3 biological replicates. (C) Colony assay in Huh7 and HepG2 cells with control or PC4 stable overexpression. Data are generated from n = 3 biological replicates. (D) EdU immunofluorescent staining performed in cells stably expressing control or PC4-OV. Scale bar = 75 μm. Data are generated from n = 3 biological replicates. (E) Soft agar assay in cells with control or PC4 stable overexpression. Scale bar = 300 μm. Data are generated from n = 4 biological replicates. (F) Tumor volume in the nude mice subcutaneously injected with Huh7 cell stably expressing control or PC4. Data are generated from n = 5 biological replicates. (G) Photographs of dissected tumors and tumor weight in the nude mice subcutaneously injected with Huh7 cell stably expressing control or PC4. (H) Representative images of Ki67 (top) and PC4 (bottom) immunostaining in the nude mice subcutaneously injected with Huh7 cell stably expressing control or PC4. Scale bar = 75 μm. Data are generated from n = 5 biological replicates. All quantifications were shown as means ± SD (one-way ANOVA test); error bars represent SD. **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are available for this figure: SourceData FS5.