NR-V04 has minimal toxicity. (A) Schematic of the toxicity testing. Male and female mice were treated with two doses of 2 mg/kg NR-V04 and two doses of 5 mg/kg NR-V04 over 2 wk. Blood samples were collected on day 0, 7, 14, and 42 for hematology analysis, and body weight was measured twice per week. On day 42, all mice were euthanized, and tissues (kidney, liver, and small intestine) were harvested for H&E staining. (B) Mice did not experience significant weight loss with NR-V04 treatment during the 42-day period. Three biological repeats, two experiments. (C–G) Hematology analysis of different blood cell components after NR-V04 or vehicle treatment, including (C) whole blood cells, (D) lymphocytes, (E) neutrophils, (F) red blood cells, and (G) platelets. Three biological repeats, two experiments. (B–G) P > 0.05. Two-sided unpaired t test was performed. (H) NR-V04 impacts on tissue histology, including representative kidney, liver, and small intestine. Three biological repeats, one experiment. Bar represents 300 μm for all images.