Celastrol treatment in B16F10 and MC38 models and NR-V04 treatment in Yumm1.7 model and schematic showing gating strategy for flow cytometry. (A and B) (A) B16F10 or (B) MC38 tumor–bearing mice were treated with vehicle or celastrol using the same treatment regimen as NR-V04 (Fig. 7). N = 5 for A, P = 0.0008, one experiment. N = 3 for B, P = 0.7852, one experiment. (C) Yumm1.7 (non-immunogenic Yumm cell line lineage) tumor–bearing mice were treated with vehicle or 1.8 mg/kg NR-V04. N = 8, P = 0.1175, one experiment. (D) Schematic showing gating strategy for flow cytometry of immune cell populations. Two-way ANOVA was performed for all tumor growth curves with P values indicated. Supplementary to Figs. 7 and 8.