The design and synthesis of NR4A1 PROTACs. (A) A docking study revealed the carboxylic acid in celastrol is a potential tethering vector for PROTAC construction. (B) The structure of synthesized PROTACs. (C and D) The initial screening of NR4A1 degradation in CHL-1 cell line. CHL-1 cells were treated with 250 nM PROTACs for 16 h and the degradation was determined by (C) immunoblotting and (D) densitometry from two experiments. Source data are available for this figure: SourceData F3.