NR4A1 expression is inversely correlated with effector molecules required for T cell activation. (A–C) NR4A1 expression in transcripts per million (TPM) is inversely correlated with (A) IFNG, (B) GZMB, and (C) PRF1 gene expression in the TCGA melanoma datasets. (D–G) GSEA analysis showing the enrichment of immune activation pathways in the melanoma specimens with low NR4A1 expression. The TCGA human melanoma dataset was divided into tertiles based on NR4A1 expression. GSEA analysis was performed using the highest tertile versus the lowest tertile of NR4A1 expression. Supplementary to Fig. 1.