Figure 5.

Lack of microtubule-binding does not affect Katanin MEI-1●MEI-2 localization during female meiosis. (A) Representative spinning disk images of fixed meiotic embryos expressing sGFP::MEI-2R WT, 6KA, and 6KD mutants exposed to mei-2(RNAi). Embryos (n = 5/5) were immunostained with anti-GFP (green), anti-tubulin (yellow), anti-MEI-1 (magenta) antibodies and colored with DAPI (blue). (B) Schematic representation of localization of the different colors at the meiotic spindle region for WT and variants.

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